Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry.

@article{Rey2009RecombinantIR,
  title={Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry.},
  author={Martial Rey and P. Man and G. Brandolin and E. Forest and L. P{\'e}losi},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2009},
  volume={23 21},
  pages={
          3431-8
        }
}
  • Martial Rey, P. Man, +2 authors L. Pélosi
  • Published 2009
  • Chemistry, Medicine
  • Rapid communications in mass spectrometry : RCM
  • Hydrogen/deuterium (H/D) exchange coupled to mass spectrometry is nowadays routinely used to probe protein interactions or conformational changes. The method has many advantages, e.g. very low sample consumption, but offers limited spatial resolution. One way to higher resolution leads through the use of different proteases or their combinations. In the present work we describe recombinant production, purification and use of aspartic protease zymogen from Rhizopus chimensis, protease type XVIII… CONTINUE READING
    46 Citations
    High-Resolution HDX-MS of Cytochrome c Using Pepsin/Fungal Protease Type XIII Mixed Bed Column
    • Y. Hamuro, T. Zhang
    • Chemistry, Medicine
    • Journal of The American Society for Mass Spectrometry
    • 2018
    • 2
    • Highly Influenced
    • PDF
    Accessing the reproducibility and specificity of pepsin and other aspartic proteases.
    • 82
    Limited proteolysis via millisecond digestions in protease-modified membranes.
    • 27
    Fragmentation hydrogen exchange mass spectrometry: a review of methodology and applications.
    • Ansgar Brock
    • Chemistry, Medicine
    • Protein expression and purification
    • 2012
    • 26

    References

    SHOWING 1-10 OF 23 REFERENCES
    Determination of amide hydrogen exchange by mass spectrometry: A new tool for protein structure elucidation
    • Z. Zhang, D. Smith
    • Chemistry, Medicine
    • Protein science : a publication of the Protein Society
    • 1993
    • 837
    • PDF
    Recombinant rhizopuspepsinogen. Expression, purification, and activation properties of recombinant rhizopuspepsinogens.
    • 30
    Hydrogen Exchange-Mass Spectrometry
    • 143
    • PDF