Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors.

  title={Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors.},
  author={Minoru Kobayashi and Joseph H. Laver and Takashi Kato and Hiroshi Miyazaki and Makio Ogawa},
  volume={86 7},
We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst-promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less… CONTINUE READING
45 Citations
39 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 45 extracted citations


Publications referenced by this paper.
Showing 1-10 of 39 references

Purification and characterization of thrombopoietin

  • T Kat, K Ogami, +13 authors H Miyazaki
  • J Biochem 118:229,
  • 1995

Assay for human blast cell colonies, in Freshney RI, Pragnell IB, Freshney MG (eds): Culture of hematopoietic cells

  • AG Leary, M Ogawa
  • 1994

Goeddel DV , Eaton DL : Stimulation of megakaqocytopoiesis and thrombopoiesis by the cMpl ligand

  • FJ deSauvage, PE Hass, +8 authors B Hultgren
  • Nature
  • 1994

Isolation and cloning of a novel human thrombopoietic factor

  • H Miyazaki, T Kat, +10 authors T Sudo
  • Exp Hematol 22:838a,
  • 1994

Similar Papers

Loading similar papers…