Recombinant human mast-cell chymase: an improved procedure for expression in Pichia pastoris and purification of the highly active enzyme.

Abstract

Human mast-cell chymase (EC 3.4.21.39) is a chymotrypsin-like serine protease that is stored in and released from mast-cell granules. This enzyme has been expressed in Pichia pastoris by homologous recombination of the cDNA coding for the mature active chymase into the Pichia genome. Cells producing the highest levels of recombinant human chymase were… (More)

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@article{Lockhart2005RecombinantHM, title={Recombinant human mast-cell chymase: an improved procedure for expression in Pichia pastoris and purification of the highly active enzyme.}, author={Brent E. Lockhart and Jessica R Vencill and Cherise M Felix and David A. Johnson}, journal={Biotechnology and applied biochemistry}, year={2005}, volume={41 Pt 1}, pages={89-95} }