Recombinant human epidermal growth factor inclusion body solubilization and refolding at large scale using expanded-bed adsorption chromatography from Escherichia coli.

@article{Sharma2008RecombinantHE,
  title={Recombinant human epidermal growth factor inclusion body solubilization and refolding at large scale using expanded-bed adsorption chromatography from Escherichia coli.},
  author={Krishna Sharma and P V Cherish Babu and P. V. K. Sasidhar and Venkatesh Srinivas and Vadrevu Krishna Mohan and E. Krishna},
  journal={Protein expression and purification},
  year={2008},
  volume={60 1},
  pages={7-14}
}
Amongst the various endogenous growth factors, epidermal growth factor (EGF) plays an important role in normal wound healing of tissue such as skin, cornea and gastrointestinal tract. Various studies have proved that supplementing recombinant human EGF (rhEGF) results in significant augmentation of wound healing. In the present work, a high level expression system with poly-arginine sequences was used for the production of recombinant human EGF (rhEGF) as inclusion bodies. The inclusion bodies… CONTINUE READING
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