Recombinant green fluorescent protein-expressing human cytomegalovirus as a tool for screening antiviral agents.

@article{Marschall2000RecombinantGF,
  title={Recombinant green fluorescent protein-expressing human cytomegalovirus as a tool for screening antiviral agents.},
  author={Manfred Marschall and Martina Freitag and Stefan Weiler and Gabriele Sorg and Thomas Stamminger},
  journal={Antimicrobial agents and chemotherapy},
  year={2000},
  volume={44 6},
  pages={
          1588-97
        }
}
A recombinant human cytomegalovirus (AD169-GFP) expressing green fluorescent protein was generated by homologous recombination. Infection of human fibroblast cultures with AD169-GFP virus produced stable and readily detectable amounts of GFP signals which were quantitated by automated fluorometry. Hereby, high levels of sensitivity and reproducibility could be achieved, compared to those with the conventional plaque reduction assay. Antiviral activities were determined for four reference… CONTINUE READING

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