Recombinant expression and inhibition mechanism analysis of pectin methylesterase from Aspergillus flavus.

Abstract

Phytopathogenic microorganisms can produce pectin methylesterase (PME) to degrade plant cell walls during plant invasion. This enzyme is thought to be a virulence factor of phytopathogens. In this work, PME from Aspergillus flavus (AFPME) was expressed in Pichia pastoris and an in vitro inhibitor study was performed. The purified AFPME with a yield of 52.2… (More)
DOI: 10.1111/1574-6968.12446

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