Recombinant expression and characterization of Thermoanaerobacter tengcongensis thermostable alpha-glucosidase with regioselectivity for high yield isomaltooligosaccharides synthesis.

@article{Zhou2009RecombinantEA,
  title={Recombinant expression and characterization of Thermoanaerobacter tengcongensis thermostable alpha-glucosidase with regioselectivity for high yield isomaltooligosaccharides synthesis.},
  author={Cheng Zhou and Yanfen Xue and Yueling Zhang and Yan Zeng and Yanhe Ma},
  journal={Journal of microbiology and biotechnology},
  year={2009},
  volume={19 12},
  pages={1547-56}
}
A novel thermostable alpha-glucosidase (TtGluA) from Thermoanaerobacter tengcongensis MB4 was successfully expressed in E. coli and characterized. The TtgluA gene contained 2253 bp, which encodes 750 amino acids. The native TtGluA was a trimer with monomer molecular mass of 89 kDa shown by SDS-PAGE. The purified recombinant enzyme showed hydrolytic activity on maltooligosaccharides, pnitrophenyl-alpha-D-glucopyranide, and dextrin with an exo-type cleavage manner. TtGluA showed preference for… CONTINUE READING
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Isomaltooligosaccharides, pp. 63-74

  • K. Buchholz, J. Seibel
  • ACS Symposium Series,
  • 2003
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