Recombinant expression and characterization of a novel endoglucanase from Bacillus subtilis in Escherichia coli

  title={Recombinant expression and characterization of a novel endoglucanase from Bacillus subtilis in Escherichia coli},
  author={Muddassar Zafar and Sibtain Ahmed and Muhammad Imran M. Khan and Amer Jamil},
  journal={Molecular Biology Reports},
The goal of this work was to produce high levels of endoglucanase in Escherichia coli for its potential usage in different industrial applications. Endoglucanase gene was amplified from genomic DNA of Bacillus subtilis JS2004 by PCR. The isolated putative endoglucanase gene consisted of an open reading frame of 1,701 nucleotides and encoded a protein of 567 amino acids with a molecular mass of 63-kDa. The gene was cloned into pET-28a(+) and expressed in E. coli BL21 (DE3). Optimum temperature… 
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