Recombinant expression, molecular characterization and crystal structure of antitumor enzyme, L-lysine α-oxidase from Trichoderma viride.

@article{Amano2015RecombinantEM,
  title={Recombinant expression, molecular characterization and crystal structure of antitumor enzyme, L-lysine α-oxidase from Trichoderma viride.},
  author={Marie Amano and Haruka Mizuguchi and Tadahisa Sano and Hiroki Kondo and Kengo Shinyashiki and Junko Inagaki and Takashi Tamura and Tatsuya Kawaguchi and Hitoshi Kusakabe and Katsumi Imada and Kenji Inagaki},
  journal={Journal of biochemistry},
  year={2015},
  volume={157 6},
  pages={549-59}
}
L-Lysine α-oxidase (LysOX) from Trichoderma viride is a homodimeric 112 kDa flavoenzyme that catalyzes the oxidative deamination of L-lysine to form α-keto-ε-aminocaproate. LysOX severely inhibited growth of cancer cells but showed relatively low cytotoxicity for normal cells. We have determined the cDNA nucleotide sequence encoding LysOX from T. viride… CONTINUE READING