Recombinant aequorin as a probe for cytosolic free Ca2+ in Escherichia coli.

@article{Knight1991RecombinantAA,
  title={Recombinant aequorin as a probe for cytosolic free Ca2+ in Escherichia coli.},
  author={Marc R. Knight and Anthony K. Campbell and Sean M. Smith and Anthony Trewavas},
  journal={FEBS letters},
  year={1991},
  volume={282 2},
  pages={405-8}
}
We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca2+]i) using recombinant aequorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca2+]i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement. 

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 21 extracted citations

Plant organellar calcium signalling: an emerging field.

Journal of experimental botany • 2012
View 1 Excerpt

Similar Papers

Loading similar papers…