Recombinant TBP, transcription factor IIB, and RAP30 are sufficient for promoter recognition by mammalian RNA polymerase II.

@article{Killeen1992RecombinantTT,
  title={Recombinant TBP, transcription factor IIB, and RAP30 are sufficient for promoter recognition by mammalian RNA polymerase II.},
  author={Marie T Killeen and Benoit Coulombe and Jack Greenblatt},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 14},
  pages={9463-6}
}
Initiation of transcription by RNA polymerase II is a complex, multistep process which requires several accessory factors in addition to the polymerase itself. A critical event in transcription initiation is the specific association of RNA polymerase II with promoter DNA. In this report we show that three eukaryotic polypeptides, produced in Escherichia coli and purified to near homogeneity, constitute a minimal set of general transcription factors both necessary and sufficient for specific and… CONTINUE READING
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