Recombinant Streptodornase Production in Escherichia . coli

  title={Recombinant Streptodornase Production in Escherichia . coli},
  author={Mahmoud Kamani and Hamid Abtahi and Razieh Nazari and Iraj Pakzad},
Introduction: In pyoderma infections, pus of density related to desoxiribonucleoproteins. streptodornase(sd) combination with streptokinase dissolve purulent secretions from infections.This study aims to produce recombinant streptodornase of Streptococcus pyogenes in E.coli. Methods: by using specific primers streptodornase gene (sd) was amplified. The constructed vector pGEX4T1-sd was transformed in E. coli BL21 (DE3)/ pLYS-S and induced with IPTG. Recombinant protein was purified by affinity… CONTINUE READING

3 Figures & Tables

Connections & Topics

Mentioned Connections BETA
Introduction : In pyoderma infections , pus of density related to desoxiribonucleoproteins .
All Topics