Recombinant Spidroins Fully Replicate Primary Mechanical Properties of Natural Spider Silk.

@article{Bowen2018RecombinantSF,
  title={Recombinant Spidroins Fully Replicate Primary Mechanical Properties of Natural Spider Silk.},
  author={Christopher Hyde Bowen and Bin Dai and Cameron J Sargent and Wenqin Bai and Pranay Ladiwala and H Feng and Wenwen Huang and David Lee Kaplan and Jonathan M. Galazka and Fuzhong Zhang},
  journal={Biomacromolecules},
  year={2018},
  volume={19 9},
  pages={
          3853-3860
        }
}
Despite significant efforts to engineer their heterologous production, recombinant spider silk proteins (spidroins) have yet to replicate the unparalleled combination of high strength and toughness exhibited by natural spider silks, preventing their use in numerous mechanically demanding applications. To overcome this long-standing challenge, we have developed a synthetic biology approach combining standardized DNA part assembly and split intein-mediated ligation to produce recombinant… 
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