Recombinant Sendai virus provides a highly efficient gene transfer into human cord blood-derived hematopoietic stem cells

@article{Jin2003RecombinantSV,
  title={Recombinant Sendai virus provides a highly efficient gene transfer into human cord blood-derived hematopoietic stem cells},
  author={Cheng-hao Jin and Koichi Kusuhara and Yoshikazu Yonemitsu and Akihiko Nomura and Shinji Okano and H Takeshita and Mamoru Hasegawa and Katsuo Sueishi and Toshiro Hara},
  journal={Gene Therapy},
  year={2003},
  volume={10},
  pages={272-277}
}
Hematopoietic stem cells (HSCs) are a promising target for gene therapy, however, the low efficiencies of gene transfer using currently available vectors face practical limitations. We have recently developed a novel and efficient gene transfer agent, namely recombinant Sendai virus (SeV), and we have here characterized SeV-mediated gene transfer to human cord blood (CB) HSCs and primitive progenitor cells (PPC) using the jelly fish green fluorescent protein (GFP) gene. Even at a relatively low… 
Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved
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Using the nonintegrating Sendai virus (SeV) vector to introduce the green fluorescent protein (GFP) gene into non-human primate cynomolgus ES cells will be a useful tool for efficient gene transfer into primate ES cells and the method of using antiviral drugs should allow further investigation for regulated SeV-mediated gene expression.
In vivo repopulation of cytoplasmically gene transferred hematopoietic cells by temperature-sensitive mutant of recombinant Sendai viral vector.
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Induction of Human Pluripotent Stem Cells by the Sendai Virus Vector: Establishment of a Highly Efficient and Footprint-Free System
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A more controllable method to eliminate the vector/transgenes from generated iPSCs has been developed by introducing several temperature-sensitive mutations into the SeV vector backbone.
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TLDR
It is proposed that the SeV vector system may prove to be a potentially important alternative in the area of T-cell-directed gene therapy used in the clinical setting.
Recombinant Sendai Virus-Mediated Gene Transfer to Adipose Tissue-Derived Stem Cells (ASCs)
TLDR
This is the first report describing the successful use of SeV-mediated gene transfer in ASCs, and the results indicate that SeV may thus provide advantages with respect to safety issues in gene therapy.
Optimization of adenovirus serotype 35 vectors for efficient transduction in human hematopoietic progenitors: comparison of promoter activities
TLDR
The construction of an optimized Ad35 vector backbone for efficient transduction into HSCs/progenitors is indicated, with the CA promoter-mediated GFP-expressing cells differentiated into progenitor cells of all lineages.
Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors
TLDR
It is shown that Sendai virus vector, an RNA virus vector that carries no risk of integrating into the host genome, is a practical solution for the efficient generation of safer iPSCs and suggested that generation of transgene-free iPSCS from cord blood cells should be an important step in providing allogeneic iPSC-derived therapy in the future.
Genetic modification of hematopoietic stem cells with nonviral systems: past progress and future prospects
TLDR
It is argued that episomally maintained self-replicating vectors combined with physical methods of delivery show the greatest promise among nonviral gene transfer strategies for the treatment of disorders of the hematopoietic system.
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