Recombinant NeutraLite avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties.

@article{Marttila2000RecombinantNA,
  title={Recombinant NeutraLite avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties.},
  author={Ari T Marttila and Olli H. Laitinen and Kari J. Airenne and Tikva Kulik and Edward A Bayer and Meir Wilchek and Markku S. Kulomaa},
  journal={FEBS letters},
  year={2000},
  volume={467 1},
  pages={31-6}
}
A recombinant non-glycosylated and acidic form of avidin was designed and expressed in soluble form in baculovirus-infected insect cells. The mutations were based on the same principles that guided the design of the chemically and enzymatically modified avidin derivative, known as NeutraLite Avidin. In this novel recombinant avidin derivative, five out of the eight arginine residues were replaced with neutral amino acids, and two of the lysine residues were replaced by glutamic acid. In… CONTINUE READING

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