Recombinant Human and Rat Ciliary Neurotrophic Factors
@article{Masiakowski1991RecombinantHA, title={Recombinant Human and Rat Ciliary Neurotrophic Factors}, author={Piotr Masiakowski and H X Liu and Czeslaw H Radziejewski and Friedrich Lottspeich and Walter Dr Oberthuer and Vivien Wong and Ronald M. Lindsay and Mark E. Furth and Nikos Panayotatos}, journal={Journal of Neurochemistry}, year={1991}, volume={57} }
The human ciliary neurotrophic factor (CNTF) gene was identified and cloned, based on homology with the recently cloned rat cDNA. The gene encodes a protein of 200 amino acids, which shares about 80% sequence identity with rat and rabbit CNTF and, like these homologues, lacks an apparent secretion signal sequence. The human CNTF gene, like the rat gene, appears to contain a single intron separating two protein coding exons. An intronless human CNTF gene was constructed by the use of polymerase…
91 Citations
Preparation and a structure-function analysis of human ciliary neurotrophic factor
- BiologyNeuroscience Research
- 1995
Establishment and characterization of a CHO cell line producing a secreted form of human ciliary neurotrophic factor: neuroprotective effects of the recombinant protein.
- BiologyBrain research. Molecular brain research
- 1994
cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene
- Biology, ChemistryNeuroscience Letters
- 1995
Structure-function studies of human ciliary neurotrophic factor
- Biology, ChemistryNeurochemical Research
- 2004
Loss of biological activity was accompanied by changes in the alphahelical nature of CNTF as measured by circular dichroism, which strengthens the proposed similarity between CNTF and the family of hematopoietic cytokines.
Preparation and biological properties of native and recombinant ciliary neurotrophic factor.
- BiologyJournal of neurobiology
- 1992
With recombinant DNA technology, rat CNTF has been synthesized in Escherichia coli, purified without denaturating agents, and found to be bioactive at a slightly lower concentration than CNTF extracted from rabbit sciatic nerves.
Production and characterization of monoclonal antibodies to human ciliary neurotrophic factor with defined epitope recognition.
- BiologyHybridoma
- 1993
Ten mouse hybridoma lines producing monoclonal antibodies against recombinant human ciliary neurotrophic factor (rhCNTF) will be useful for studying the distribution of CNTF in the nervous system and in developing an enzyme-linked immunosorbent assay for the quantitative determinations ofCNTF in various neuropathologies.
Characterisation of high-affinity and low-affinity receptors for ciliary neurotrophic factor.
- Biology, ChemistryEuropean journal of biochemistry
- 1993
Antisense experiments confirmed that CNTFR alpha is necessary for high affinity binding of 125I-CNTF and therefore a necessary subunit of the high-affinity receptor.
Neurotrophic Factor Receptors and Their Signal Transduction Capabilities in Rat Astrocytes
- BiologyThe European journal of neuroscience
- 1994
It is shown that, both in vivo and in vitro, astrocytes express receptors for two separate families of neurotrophic factors, which may act primarily to sequester or present the neurotrophins, whereas in the case of CNTF a functional response can be initiated within theAstrocyte.
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