Recombinant Human and Rat Ciliary Neurotrophic Factors

@article{Masiakowski1991RecombinantHA,
  title={Recombinant Human and Rat Ciliary Neurotrophic Factors},
  author={Piotr Masiakowski and H X Liu and Czeslaw H Radziejewski and Friedrich Lottspeich and Walter Dr Oberthuer and Vivien Wong and Ronald M. Lindsay and Mark E. Furth and Nikos Panayotatos},
  journal={Journal of Neurochemistry},
  year={1991},
  volume={57}
}
The human ciliary neurotrophic factor (CNTF) gene was identified and cloned, based on homology with the recently cloned rat cDNA. The gene encodes a protein of 200 amino acids, which shares about 80% sequence identity with rat and rabbit CNTF and, like these homologues, lacks an apparent secretion signal sequence. The human CNTF gene, like the rat gene, appears to contain a single intron separating two protein coding exons. An intronless human CNTF gene was constructed by the use of polymerase… 
Structure-function studies of human ciliary neurotrophic factor
TLDR
Loss of biological activity was accompanied by changes in the alphahelical nature of CNTF as measured by circular dichroism, which strengthens the proposed similarity between CNTF and the family of hematopoietic cytokines.
Preparation and biological properties of native and recombinant ciliary neurotrophic factor.
TLDR
With recombinant DNA technology, rat CNTF has been synthesized in Escherichia coli, purified without denaturating agents, and found to be bioactive at a slightly lower concentration than CNTF extracted from rabbit sciatic nerves.
Ciliary neurotrophic factor: a review.
Production and characterization of monoclonal antibodies to human ciliary neurotrophic factor with defined epitope recognition.
TLDR
Ten mouse hybridoma lines producing monoclonal antibodies against recombinant human ciliary neurotrophic factor (rhCNTF) will be useful for studying the distribution of CNTF in the nervous system and in developing an enzyme-linked immunosorbent assay for the quantitative determinations ofCNTF in various neuropathologies.
Characterisation of high-affinity and low-affinity receptors for ciliary neurotrophic factor.
TLDR
Antisense experiments confirmed that CNTFR alpha is necessary for high affinity binding of 125I-CNTF and therefore a necessary subunit of the high-affinity receptor.
Neurotrophic Factor Receptors and Their Signal Transduction Capabilities in Rat Astrocytes
TLDR
It is shown that, both in vivo and in vitro, astrocytes express receptors for two separate families of neurotrophic factors, which may act primarily to sequester or present the neurotrophins, whereas in the case of CNTF a functional response can be initiated within theAstrocyte.
Comparison of solution properties of human and rat ciliary neurotrophic factor.
TLDR
The solution structure and stability of rat and human ciliary neurotrophic factor (CNTF) were examined by circular dichroism, Fourier transform infrared (FTIR) and fluorescence spectroscopy and sedimentation equilibrium analyses, demonstrating that bothRat and human CNTF exist primarily as monomers.
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References

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The complementary DNA-deduced amino-acid sequence and subsequent expression of cDNA clones covering the entire coding region in HeLa-cells indicate that CNTF is a cytosolic protein, and shows thatCNTF is not a target-derived neurotrophic factor.
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TLDR
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TLDR
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