Recombinant Human and Rat Ciliary Neurotrophic Factors

  title={Recombinant Human and Rat Ciliary Neurotrophic Factors},
  author={Piotr Masiakowski and H X Liu and Czeslaw H Radziejewski and Friedrich Lottspeich and Walter Dr Oberthuer and Vivien Wong and Ronald M. Lindsay and Mark E. Furth and Nikos Panayotatos},
  journal={Journal of Neurochemistry},
The human ciliary neurotrophic factor (CNTF) gene was identified and cloned, based on homology with the recently cloned rat cDNA. The gene encodes a protein of 200 amino acids, which shares about 80% sequence identity with rat and rabbit CNTF and, like these homologues, lacks an apparent secretion signal sequence. The human CNTF gene, like the rat gene, appears to contain a single intron separating two protein coding exons. An intronless human CNTF gene was constructed by the use of polymerase… 
Structure-function studies of human ciliary neurotrophic factor
Loss of biological activity was accompanied by changes in the alphahelical nature of CNTF as measured by circular dichroism, which strengthens the proposed similarity between CNTF and the family of hematopoietic cytokines.
Preparation and biological properties of native and recombinant ciliary neurotrophic factor.
With recombinant DNA technology, rat CNTF has been synthesized in Escherichia coli, purified without denaturating agents, and found to be bioactive at a slightly lower concentration than CNTF extracted from rabbit sciatic nerves.
Ciliary neurotrophic factor: a review.
Production and characterization of monoclonal antibodies to human ciliary neurotrophic factor with defined epitope recognition.
Ten mouse hybridoma lines producing monoclonal antibodies against recombinant human ciliary neurotrophic factor (rhCNTF) will be useful for studying the distribution of CNTF in the nervous system and in developing an enzyme-linked immunosorbent assay for the quantitative determinations ofCNTF in various neuropathologies.
Characterisation of high-affinity and low-affinity receptors for ciliary neurotrophic factor.
Antisense experiments confirmed that CNTFR alpha is necessary for high affinity binding of 125I-CNTF and therefore a necessary subunit of the high-affinity receptor.
Neurotrophic Factor Receptors and Their Signal Transduction Capabilities in Rat Astrocytes
It is shown that, both in vivo and in vitro, astrocytes express receptors for two separate families of neurotrophic factors, which may act primarily to sequester or present the neurotrophins, whereas in the case of CNTF a functional response can be initiated within theAstrocyte.
Comparison of solution properties of human and rat ciliary neurotrophic factor.
The solution structure and stability of rat and human ciliary neurotrophic factor (CNTF) were examined by circular dichroism, Fourier transform infrared (FTIR) and fluorescence spectroscopy and sedimentation equilibrium analyses, demonstrating that bothRat and human CNTF exist primarily as monomers.


Molecular cloning, expression and regional distribution of rat ciliary neurotrophic factor
The complementary DNA-deduced amino-acid sequence and subsequent expression of cDNA clones covering the entire coding region in HeLa-cells indicate that CNTF is a cytosolic protein, and shows thatCNTF is not a target-derived neurotrophic factor.
Purification, cloning, and expression of ciliary neurotrophic factor (CNTF).
CNTF is a neural effector without significant sequence homologies to any previously reported protein and has been transiently expressed from a rabbit complementary DNA clone.
Molecular cloning and expression of brain-derived neurotrophic factor
The full primary structure of brain-derived neurotrophic factor is reported and it is established that these two neurotrophic factors are related both functionally and structurally.
Neurotrophin-3: a neurotrophic factor related to NGF and BDNF.
The distribution of NT-3 messenger RNA and its biological activity on a variety of neuronal populations clearly distinguishNT-3 from NGF and BDNF, and provide compelling evidence that NT- 3 is an authentic neurotrophic factor that has its own characteristic role in vivo.
Purification of the Chick Eye Ciliary Neuronotrophic Factor
Purified CNTF represents the first purified neuronotrophic factor which addresses parasympathetic cholinergic neurons and supports the 24‐h survival of cultured neurons from certain chick and rodent sensory and sympathetic ganglia.
Ciliary neurotrophic factor induces cholinergic differentiation of rat sympathetic neurons in culture
The effects of CNTF on ChAT and TH are not due to selective survival of cells that acquire cholinergic traits in vitro, but are rather due to the induction of cholinergy differentiation of noradrenergic sympathetic neurons.
Novel neurotrophic factors, receptors, and oncogenes.
  • P. Walicke
  • Biology
    Annual review of neuroscience
  • 1989
A potential application for NTFs in treatment of CNS disease was suggested by recent studies in which exogeneous NGF was shown to increase survival of septal neurons after lesions of the fornix and to reverse the atrophic changes associated with aging in the basal nucleus.