Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines.

@article{Khurana2011RecombinantHP,
  title={Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines.},
  author={Surender Khurana and Christopher Larkin and Swati Verma and Manju B. Joshi and Juan M. Fontana and Alasdair C. Steven and Lisa R. King and Jody Manischewitz and William McCormick and Rajesh Kumar Gupta and H. Golding},
  journal={Vaccine},
  year={2011},
  volume={29 34},
  pages={
          5657-65
        }
}
Vaccine production and initiation of mass vaccination is a key factor in rapid response to new influenza pandemic. During the 2009-2010 H1N1 pandemic, several bottlenecks were identified, including the delayed availability of vaccine potency reagents. Currently, antisera for the single-radial immunodiffusion (SRID) potency assay are generated in sheep immunized repeatedly with HA released and purified after bromelain-treatment of influenza virus grown in eggs. This approach was a major… CONTINUE READING
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