Recombinant Bacterial Expression of the Lysozyme from the Tobacco-Hornworm Manduca sexta with Activity at Low Temperatures

@article{GarcaOrozco2005RecombinantBE,
  title={Recombinant Bacterial Expression of the Lysozyme from the Tobacco-Hornworm Manduca sexta with Activity at Low Temperatures},
  author={Karina D. Garc{\'i}a-Orozco and Alonso Alexis L{\'o}pez-Zavala and Daniel Puentes-Camacho and Ana Mar{\'i}a Calder{\'o}n-de-la-Barca and Rogerio R Sotelo-Mundo},
  journal={Biotechnology Letters},
  year={2005},
  volume={27},
  pages={1075-1080}
}
A gene coding for lysozyme from the insect Manduca sexta (Ms-lyz) was expressed in Escherichia coli. The protein was produced as an insoluble cytoplasmic inclusion body which was denatured in 8 M guanidine-HCl, renatured and purified by affinity and ion-exchange chromatography. The N-terminal sequence and the activity of the recombinant protein against Micrococcus luteus confirmed that correct expression had occurred. When Ms-lyz activity was compared to hen egg white lysozyme, the insect… CONTINUE READING