Recognition specificity for the bacterial avirulence protein AvrPto is determined by Thr-204 in the activation loop of the tomato Pto kinase.

@article{Frederick1998RecognitionSF,
  title={Recognition specificity for the bacterial avirulence protein AvrPto is determined by Thr-204 in the activation loop of the tomato Pto kinase.},
  author={Reid D. Frederick and R. L. Thilmony and Guido Sessa and Gregory B Martin},
  journal={Molecular cell},
  year={1998},
  volume={2 2},
  pages={241-5}
}
The Pto kinase confers resistance in tomato to P. syringae pv. tomato strains expressing the AvrPto protein. Physical interaction of the Pto kinase and AvrPto protein in the plant cell initiates host defense responses. The recognition event between these two proteins is very specific; AvrPto does not interact with other closely related kinases, including the Fen kinase, which shares 80% amino acid identity with Pto. By using Pto-Fen chimeric proteins and site-directed mutagenesis, we found that… CONTINUE READING

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