Recognition of the mRNA AU-rich element by the zinc finger domain of TIS11d


The tandem zinc finger (TZF) domain of the protein TIS11d binds to the class II AU-rich element (ARE) in the 3′ untranslated region (3′ UTR) of target mRNAs and promotes their deadenylation and degradation. The NMR structure of the TIS11d TZF domain bound to the RNA sequence 5′-UUAUUUAUU-3′ comprises a pair of novel CCCH fingers of type CX8CX5CX3H separated by an 18-residue linker. The two TIS11d zinc fingers bind in a symmetrical fashion to adjacent 5′-UAUU-3′ subsites on the single-stranded RNA via a combination of electrostatic and hydrogen-bonding interactions, with intercalative stacking between conserved aromatic side chains and the RNA bases. Sequence specificity in RNA recognition is achieved by a network of intermolecular hydrogen bonds, mostly between TIS11d main-chain functional groups and the Watson-Crick edges of the bases. The TIS11d structure provides insights into the RNA-binding functions of this large family of CCCH zinc finger proteins.

DOI: 10.1038/nsmb738
Citations per Year

720 Citations

Semantic Scholar estimates that this publication has 720 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Hudson2004RecognitionOT, title={Recognition of the mRNA AU-rich element by the zinc finger domain of TIS11d}, author={Brian P. Hudson and Maria A. Martinez-Yamout and H Jane Dyson and Peter E. Wright}, journal={Nature Structural &Molecular Biology}, year={2004}, volume={11}, pages={257-264} }