Recognition of oxidized abasic sites by repair endonucleases.

@article{Hring1994RecognitionOO,
  title={Recognition of oxidized abasic sites by repair endonucleases.},
  author={M H{\"a}ring and Harold R{\"u}diger and Bruce Demple and S. Boiteux and Bernd Epe},
  journal={Nucleic acids research},
  year={1994},
  volume={22 11},
  pages={2010-5}
}
The recognition of 'regular' and 'oxidized' sites of base loss (AP sites) in DNA by various AP endonucleases was compared. Model substrates with regular AP sites (resulting from mere hydrolysis of the glycosylic bond) were produced by damaging bacteriophage PM2 DNA by exposure to low pH; those with AP sites oxidized at the C-4'- and C-1'-position of the sugar moiety by exposure to Fe(III)-bleomycin in the presence of H2O2 and to Cu(II)-phenanthroline in the presence of H2O2 and ethanol… CONTINUE READING