Corpus ID: 21637638

Recognition of a CD4+ mouse medullary thymocyte subpopulation by Amaranthus leucocarpus lectin.

  title={Recognition of a CD4+ mouse medullary thymocyte subpopulation by Amaranthus leucocarpus lectin.},
  author={R. Lascura{\'i}n and R. Ch{\'a}vez and P. Gorocica and A. P{\'e}rez and L. Monta{\~n}o and E. Zenteno},
  volume={83 3},
We have used the Gal beta(1-->3)GalNAc-specific Amaranthus leucocarpus lectin to isolate a thymus cell subpopulation which is different from that sorted with Arachis hypogaea lectin. The cells recognized by A. leucocarpus lectin were predominantly CD4+, whereas a minor proportion of CD8+ cells (approximately 11%) were also identified. The A. leucocarpus-positive cells were located in the thymus medulla and the cortico-medullary junction. The cortex was negative for A. leucocarpus cells. 
Amaranthus leucocarpus lectin recognizes human naive T cell subpopulations.
Amaranthus leucocarpus lectin (ALL) is specific for GalNAc residue found in the inner core of Gal beta 1,3GalNAc alpha 1,O-Ser/Thr disaccharide (T-antigen) or GalNAc alpha 1,O-Ser/Thr (Tn-antigen).Expand
Isolation of the receptor for Amaranthus leucocarpus lectin from murine naive thymocytes.
Ionic strength chromatography revealed that the ALL-thymocyte receptor (ALLTr) is made up of three isoforms, which possess similar amino acid composition but show slight differences in their sugar composition. Expand
Peanut and Amaranthus leucocarpus lectins discriminate between memory and naive/quiescent porcine lymphocytes.
The results suggest that the cellular distribution/organization of the O-glycosydically linked glycans on lymphocytes may correlate with biological functions, and that PNA could be a tool to isolate specifically porcine memory T cell subsets, whereas ALL could be useful to isolate naive/quiescent T lymphocytes. Expand
O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin
Evaluating the phenotype of CD4+ T cells recognized by ALL in peripheral blood mononuclear cells obtained from healthy volunteers showed that galactose-N-acetylgalactosamine and N-galactsosamine-bearing CD4- T cells expressed phenotypic markers of NnTreg cells. Expand
The Amaranthus leucocarpus lectin enhances the anti-CD3 antibody-mediated activation of human peripheral blood CD4+ T cells.
Findings indicate that lectin from Amaranthus leucocarpus seeds (ALL) binds to human activated CD4(+) T cells and enhances the degree of activation of CD3-type T cells that are stimulated with anti-CD3 antibodies, providing a new tool for analyzing T cell activation mechanisms. Expand
Amaranthus leucocarpus lectin recognizes a moesin-like O-glycoprotein and costimulates murine CD3-activated CD4+ T cells
It is suggested that a moesin‐like O‐glycoprotein is the ALL‐recognized molecule in lipid rats, which induces costimulatory signals on CD4+ T cells. Expand
Isolation of the receptor for the Amaranthus leucocarpus lectin from human T lymphocytes.
The results indicated that the tryptic peptides obtained showed 54% homology with a DnaK-core molecular chaperone, 47% with human KIAA protein, and 44% with heat shock protein 8, indicating that the glycoprotein recognized by ALL is present mainly on naive or quiescent T cells. Expand
Relevance of sialoglycoconjugates in murine thymocytes during maturation and selection in the thymus.
The results suggest that in the initial stages of the differentiation pathway, a great number of thymocytes express analpha2,6 linked sialic acid on their surface and as they progress to more mature stages there is a change in the sialylation pattern to alpha2,3 linked sIALic acids probably due to a regulated expression of different sIALyltransferases, which could be modulated by the thymic microenvironment. Expand
Differential expression of a 70 kDa O-glycoprotein on T cells: a possible marker for naive and early activated murine T cells.
The results suggest that ALL detects a non-described surface O-glycoprotein selectively expressed by naive CD8(+) T cells and by early activated CD4(+)T cells. Expand
Specificity of Amaranthus leucocarpus syn. hypocondriacus lectin for O-glycopeptides.
Amaranthus leucocarpus syn. hypochondriacus lectin (ALL) has been shown to be specific for N-acetyl-D-galactosamine (GalNAc). In this work, we determined a value of 1.0 x 10(-2) M for the associationExpand