Recognition and cleavage site of the intron-encoded omega transposase.

  title={Recognition and cleavage site of the intron-encoded omega transposase.},
  author={Laurence Colleaux and L. d'Auriol and Francis Galibert and Bernard Dujon},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={85 16},
The optional group I intron of the mitochondrial 21S rRNA gene of Saccharomyces cerevisiae contains a 235-codon-long open reading frame the translation product of which (the omega transposase) catalyzes the formation of a double-strand break within the intron-minus (omega-) copies of the same gene. Purified omega transposase generates in vitro a 4-base-pair staggered cut with 3' hydroxyl overhangs at the exact position where the intron eventually inserts in the gene. Using randomly mutagenized… CONTINUE READING