Recoding structural glycoprotein E2 in classical swine fever virus (CSFV) produces complete virus attenuation in swine and protects infected animals against disease.

@article{VelzquezSalinas2016RecodingSG,
  title={Recoding structural glycoprotein E2 in classical swine fever virus (CSFV) produces complete virus attenuation in swine and protects infected animals against disease.},
  author={Lauro Vel{\'a}zquez-Salinas and Guillermo R. Risatti and Lauren G. Holinka and Vivian K O'Donnell and Jolene Carlson and Marialexia Alfano and Luis Rodriguez and C Castells Carrillo and Douglas P Gladue and Manuel V Borca},
  journal={Virology},
  year={2016},
  volume={494},
  pages={
          178-89
        }
}
Controlling classical swine fever (CSF) mainly involves vaccination with live attenuated vaccines (LAV). Experimental CSFV LAVs has been lately developed through reverse genetics using several different approaches. Here we present that codon de-optimization in the major CSFV structural glycoprotein E2 coding region, causes virus attenuation in swine. Four different mutated constructs (pCSFm1-pCSFm4) were designed using various mutational approaches based on the genetic background of the highly… CONTINUE READING