Receptor binding of asialoerythropoietin.

  title={Receptor binding of asialoerythropoietin.},
  author={Y J Dong and C K Kung and Eugene Goldwasser},
  journal={Journal of cellular biochemistry},
  volume={48 3},
The interaction of 125I-asialoerythropoietin (asialoepo) with receptors has been characterized both by binding assay and affinity cross-linking. Purified spleen cells from mice infected with the anemia strain of Friend virus (FVA cells) have receptors for 125I-asialoepo with two classes of affinity constant: one with Kd = 0.02-0.03 nM and 300-400 per cell, the other with lower affinity (Kd = 0.9-1.2 nM) and 1,000-1,200 per cell. The Kd value for the high affinity site is one-third of that for… CONTINUE READING