Receptor and protein kinase C-mediated regulation of ARF binding to the Golgi complex

@article{Matteis1993ReceptorAP,
  title={Receptor and protein kinase C-mediated regulation of ARF binding to the Golgi complex},
  author={Maria Antonietta De Matteis and Giovanna Santini and Richard A. Kahn and Giuseppe Di Tullio and Alberto Luini},
  journal={Nature},
  year={1993},
  volume={364},
  pages={818-821}
}
THE formation of constitutive transport vesicles involves the association of non-clathrin coat proteins to transport organelles1,2. Here we report that IgE receptors and protein kinase C (PKC) regulate the GTP-dependent binding of the two coat proteins ADP-ribosylation factor (ARF) and β-COP3–5 to Golgi membranes in rat basophilic leukaemia cells. Activation of IgE receptors and PKC prevented the ARF and β-COP dissociation from Golgi membranes that occurs in permeabilized cells in the absence… CONTINUE READING