Recent methodological advances in MALDI mass spectrometry

@article{Dreisewerd2014RecentMA,
  title={Recent methodological advances in MALDI mass spectrometry},
  author={Klaus Dreisewerd},
  journal={Analytical and Bioanalytical Chemistry},
  year={2014},
  volume={406},
  pages={2261-2278}
}
  • K. Dreisewerd
  • Published 2014
  • Chemistry, Medicine
  • Analytical and Bioanalytical Chemistry
AbstractMatrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for characterization of large, thermally labile biomolecules. Advantages of this analytical technique are high sensitivity, robustness, high-throughput capacity, and applicability to a wide range of compound classes. For some years, MALDI-MS has also been increasingly used for mass spectrometric imaging as well as in other areas of clinical research. Recently, several new concepts have been presented… Expand
MALDI matrices for low molecular weight compounds: an endless story?
TLDR
This review is intended to offer a concise critical overview of the most recent achievements about MALDI matrices capable of specifically address the challenging issue of small molecules analysis. Expand
Recommendations for quantitative analysis of small molecules by matrix-assisted laser desorption ionization mass spectrometry.
TLDR
This review focuses on practical aspects of modern MalDI for quantitation of small molecules conducted in an ordinary way (no special reagents, devices or techniques for the spotting step of MALDI), and includes ordinary, preferred methods. Expand
Quantification of low molecular weight compounds by MALDI imaging mass spectrometry - A tutorial review.
TLDR
Strategies for Absolute quantification by MALDI-MSI should provide an accurate value of the concentration/amount of the compound of interest in relatively small, well-defined region of interest of the examined tissue, ideally in a single pixel. Expand
MALDI-TOF MASS SPECTROMETRY IMAGING OF METALLOTHIONEIN INCHICKEN EMBRYO
In last decades the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) has become an outstanding tool for detecting spatial distribution of differentExpand
MALDI-TOF MASS SPECTROMETRY IMAGING OF METALLOTHIONEIN IN CHICKEN EMBRYO
In last decades the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) has become an outstanding tool for detecting spatial distribution of differentExpand
Mass spectrometry imaging with laser-induced postionization
TLDR
A wavelength-tunable postionization laser is used to initiate secondary MALDI-like ionization processes in the gas phase to increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot. Expand
Remote Atmospheric Pressure Infrared Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry (Remote IR-MALDI MS) of Proteins *
TLDR
The remote IR-MALDI system is demonstrated to have capability to detect and analyze peptides and proteins, and the corresponding mass spectra show ESI-like charge state distribution, opening many applications for structural elucidation to be performed in real-time by Top-Down strategy. Expand
Atmospheric pressure MALDI mass spectrometry imaging of tissues and cells at 1.4-μm lateral resolution
We report an atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) setup with a lateral resolution of 1.4 μm, a mass resolution greater thanExpand
e-MALDI: An Electrowetting-Enhanced Drop Drying Method for MALDI Mass Spectrometry.
TLDR
It is demonstrated that electrowetting-assisted drying of solutions of common MALDI matrix materials and a variety of common low molecular weight pharmaceutical molecules indeed leads to substantially smaller and more homogeneous sample spots on special electroweta-functionalized e-MALDI target plates. Expand
Mesoporous organosilica films for laser desorption/ionization mass spectrometry
Abstract Mass spectrometry (MS) is a powerful analytical tool that can detect and identify multiple kinds of compounds such as biomolecules with high sensitivity. Recently, various nanoporous solidExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 163 REFERENCES
Application of secondary ion and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for the quantitative analysis of biological molecules
This review primarily focuses on the current status of two solid-state mass spectrometric (MS) techniques, namely, static secondary-ion (SIMS) and matrix-assisted laser desorption-ionization (MALDI)Expand
Heterogeneity within MALDI samples as revealed by mass spectrometric imaging.
TLDR
MALDI-based imaging is developed as a diagnostic tool for examining the relationships between preparation strategy, sample morphology, and spectral quality and provides a wealth of unbiased information concerning sample defects, spectral reproducibility, mass accuracy, differential analyte distributions, and the validity of internal standards. Expand
Liquid ultraviolet matrix‐assisted laser desorption/ionization – mass spectrometry for automated proteomic analysis
TLDR
The use of the presented liquid matrix significantly reduces the extent of unspecific ion signals in peptide mass fingerprints compared to typically used solid matrices, such as 2,5‐dihydroxybenzoic acid (DHB) or α‐cyano‐hydroxycinnamic acid (CHCA). Expand
MALDI mass spectrometry imaging in oncology (Review).
TLDR
An overview of MALDI imaging functionality and its advantages and disadvantages is provided, and a current literature overview of malignancy-based biomarker detection is provided. Expand
MALDI in-source decay, from sequencing to imaging.
TLDR
The mechanisms involved in the ISD processes are reviewed, ISD applications like top-down sequencing and post-translational modifications (PTMs) studies are discussed, and MALDI-ISD tissue imaging applications are reviewed. Expand
Optimizing UV laser focus profiles for improved MALDI performance.
TLDR
It is shown, for the first time, that a spatially structured laser beam profile in lieu of a Gaussian profile is of striking importance and enabled us to design diode-pumped Nd : YAG lasers that on various critical applications perform as well for MALDI as the nitrogen lasers and in some respects even better. Expand
A Mechanism for Ionization of Nonvolatile Compounds in Mass Spectrometry: Considerations from MALDI and Inlet Ionization
TLDR
Data is presented suggesting that the prompt and delayed ionization reported for vacuum MALDI are both fast processes relative to producing highly charged ions by LSI, and charged droplets/droplets may be a common link for ionization of nonvolatile compounds by a variety of MS ionization methods, including LSI. Expand
Mass spectrometric imaging for biomedical tissue analysis.
TLDR
This review focuses on one of the latest, rapidly developing innovations in MS namely mass spectrometric imaging (MSI), this young technique takes benefit from all methodological and technological developments in general MS over the last decades. Expand
Label-free quantification using MALDI mass spectrometry: considerations and perspectives
TLDR
The capabilities of LC–MALDI MS systems have not yet been fully utilized because of a lack of suitable software tools, and the potential of state-of-the-art instruments is outlined by reporting unpublished results obtained from twenty-four complex biological samples. Expand
Ion formation in MALDI mass spectrometry
The origin of ions in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is currently a matter of active research. A number of chemical and physical pathways have been suggestedExpand
...
1
2
3
4
5
...