Recent developments in the use of viability dyes and quantitative PCR in the food microbiology field.

@article{Elizaquvel2014RecentDI,
  title={Recent developments in the use of viability dyes and quantitative PCR in the food microbiology field.},
  author={Patricia Elizaqu{\'i}vel and Rosa Vercher Aznar and Gl{\`o}ria S{\'a}nchez},
  journal={Journal of applied microbiology},
  year={2014},
  volume={116 1},
  pages={1-13}
}
The increase in foodborne outbreaks highlights the need for rapid, sensitive and specific methods for food safety monitoring, enabling specific detection and quantification of viable foodborne pathogens. Real-time PCR (qPCR) combined with the use of viability dyes, recently introduced, fulfils all these requirements. The strategy relies on the use of DNA-binding molecules such as propidium monoazide (PMA) or ethidium monoazide (EMA) as sample pretreatment previous to the qPCR. These molecules… CONTINUE READING
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