Recent development in NMR methodology for the study of molecular structure and dynamics

Abstract

The usage of nuclear magnetic resonance for exploring molecular structure and molecular dynamics is reviewed. The cyclic decapeptide antmaaide is used as an example. Introduction Nuclear Magnetic Resonance (NMR) has become a very powerful and informative technique in a large variety of disciplines of natural science, reaching from solid state physics to mineralogy, all brands of chemistry, biochemistry, biology, and medicine (1-3). NMR t a k a advantage of the presence of nuclear magnetic moments that interact very weakly with the surroundings but are nevertheless very sensitive to the local environment. This distinguishes NMR from other structure-analytical techniques that explore more global properties, such as ultraviolet spectroscopy observing the electronic excitement of entire molecules, or vibrational spectroscopy measuring the normal modes of molecules. NMR can indeed, with its numerous "spies", obtain very detailed, highly localized information on molecules and materials. On the other hand, the shortsightedness of NMR is of disadvantage when long-range order must be studied. Fore these investigations, scattering techniques, such as x-ray, neutron and light scattering, are predestinate. For the determination of the three-dimensional structure of molecules in solution, there is hardly a competitive technique that would match the power of NMR. In molecular biology, NMR has become, based on the pioneering work of the research group of Professor Kurt Wiithrich, next to x-ray crystallography, the most important structuredetermination technique for medium-size proteins up to about 25 000 Dalton, for nucleic acid fragments, and for oligo-saccharides. More than hundred detailed structures have already been determined in the past 10 years. Of equal importance is the study of biomolecular interactions that leads directly to an improved understanding of biomolecular function, a subject of enormous importance at present and in the near future. It is well known that static structure or conformation is a small, although relevant aspect of the full truth. Of at least equal importance is the dynamics of molecules. Here, NMR also

Cite this paper

@inproceedings{Ernst2004RecentDI, title={Recent development in NMR methodology for the study of molecular structure and dynamics}, author={Richard R. Ernst}, year={2004} }