Recent advances in the diagnosis of dermatophytosis

  title={Recent advances in the diagnosis of dermatophytosis},
  author={Jubeda Begum and Nasir Akbar Mir and Madhu Cholenahalli Lingaraju and Bidyarani Buyamayum and Kapil Dev},
  journal={Journal of Basic Microbiology},
  pages={293 - 303}
Dermatophytosis is a disease of global significance caused by pathogenic keratinolytic fungi called dermatophytes in both animals and humans. The recent taxonomy of dermatophytes classifies them into six pathogenic genera, namely Microsporum, Trichophyton, Epidermophyton, Nannizzia, Lophophyton and Arthroderma. It is because of the delayed diagnostic nature and low accuracy of dermatophyte detection by conventional methods that paved the path for the evolution of molecular diagnostic techniques… 

Epidemiology and Diagnostic Perspectives of Dermatophytoses

Molecular techniques are promising but it is necessary to improve the quality and availability of the information in genomic and proteomic databases in order to streamline the use of bioinformatics in the identification of dermatophytes of clinical interest.

Current Topics in Dermatophyte Classification and Clinical Diagnosis

This review discusses dermatophyte classification including name changes for medically important species, current and potential diagnostic techniques for detecting dermatophytes, and an in-depth review of Microsporum canis, a prevalent zoonotic dermatophytosis.

Recent trends in rapid diagnostic techniques for dermatophytosis

The laborious, exhaustive, and time-consuming gold standard method was a real challenge facing all dermatologists to achieve a rapid, accurate dermatophytosis diagnosis, and various studies developed more rapid, accurately, reliable, sensitive, and specific diagnostic tools.

Strategies to improve the diagnosis and clinical treatment of dermatophyte infections.

Properties of natural herbal, laser, and photodynamic treatments can be used as alternative treatments in patients who cannot tolerate topical and systemic antifungal treatments, especially in immunosuppressed patients.

State-of-the-Art Dermatophyte Infections: Epidemiology Aspects, Pathophysiology, and Resistance Mechanisms

The current knowledge about dermatophyte infections is outlined, with a focus on signaling pathways required for fungal infection establishment and a broad perspective on cellular and molecular factors involved in antifungal resistance and tolerance.

Prevalence of dermatophytosis in animals and antifungal susceptibility testing of isolated Trichophyton and Microsporum species.

Direct microscopy and in vitro culture were equally efficient in diagnosis of dermatophytosis and maximum inhibition of fungal growth was exhibited by clotrimazole and least by fluconazole.

Сomparison of the main methods of clinical and laboratory diagnostics of dermatophytosis

The aim of the study was to compare different methods of clinical and laboratory diagnosis of dermatophytosis, which revealed the causative agent in 15 cases, which is 93.8%.

Rapid and Visible RPA-Cas12a fluorescence Assay for Accurate Detection of Zoonotic Dermatophytes

Compared with other methods, the RPA-Cas12a-fluorescence assay requires less time and less complicated equipment, and visible changes can be clearly observed, which is suitable for on-site clinical diagnosis.

New Insights in Dermatophytes: Microsporum spp. and Nannizzia spp.

These fungi present an increasing risk in human health care; studies in physiology, genetics and biochemistry, pathology of dermatophytosis, and immune response are essential for the development of new diagnostic measures, treatment protocols, and prevention strategies.

Trends in the epidemiology of dermatophytosis in the Middle East and North Africa region

There is an urgent need for further studies based on reliable diagnostic tools and standard susceptibility testing methods for dermatophytosis, which represents a major challenge for developing countries such as those in the Middle East and North Africa.



Molecular diagnosis of dermatophyte infections

Current molecular methods are on the verge of overcoming most of the early challenges regarding dermatophyte taxonomy, DNA extraction procedures and species specificity, and thus may lead to an increased adoption of such methods, pointing towards a novel consensus in which molecular methods supplement or even replace classical diagnosis of dermatophytosis.

Conventional Methods for the Diagnosis of Dermatophytosis

Progress is still needed for the development of biochemical or immunological tests allowing an accurate identification at the species level, pending for the availability of molecular biology-based kits.

Molecular Approaches in the Diagnosis of Dermatophytosis

Development of molecular diagnosis of dermatophytosis requires the investigation of additional molecular markers for diagnostic tools targeting multiple loci as well as the improvement of techniques.

The agony of choice in dermatophyte diagnostics-performance of different molecular tests and culture in the detection of Trichophyton rubrum and Trichophyton interdigitale.

  • C. KupschT. Ohst Y. Gräser
  • Biology
    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2016

Molecular diagnosis of onychomycosis.

Application of PCR to the identification of dermatophyte fungi.

AP-PCR provides a rapid and practical tool for identification of dermatophyte isolates that is independent of morphological and biochemical characteristics and thus enhances laboratory diagnosis of dermatphytosis.

Comparison of diagnostic methods in the evaluation of onychomycosis.

Bx/PAS is the most sensitive method and superior to the other methods in its negative predictive value and potentially is the single method of choice for the evaluation of onychomycosis.

Evaluation of a commercial PCR test for the diagnosis of dermatophyte nail infections.

Multiplex PCR offers a time-saving diagnostic tool for tinea unguium and augments laboratory assistance to clinical evaluation for proper treatment and increased species-specific detection of dermatophytes by 21.1 %, leading to a threefold increase as compared to culture alone.

Reappraisal of Conventional Diagnosis for Dermatophytes

Despite the recent availability of several PCR-based kits and an extensive literature on molecular methods allowing the detection of fungal DNA or both detection and direct identification of the main dermatophyte species, the biological diagnosis of dermatophytosis in 2016 still relies on both direct examination and cultures of appropriate clinical specimens.