Recent advances in targeted genome engineering in mammalian systems.

@article{Sun2012RecentAI,
  title={Recent advances in targeted genome engineering in mammalian systems.},
  author={Ning Sun and Zhanar Abil and Huimin Zhao},
  journal={Biotechnology journal},
  year={2012},
  volume={7 9},
  pages={1074-87}
}
Targeted genome engineering enables researchers to disrupt, insert, or replace a genomic sequence precisely at a predetermined locus. One well-established technology to edit a mammalian genome is known as gene targeting, which is based on the homologous recombination (HR) mechanism. However, the low HR frequency in mammalian cells (except for mice) prevents its wide application. To address this limitation, a custom-designed nuclease is used to introduce a site-specific DNA double-strand break… CONTINUE READING
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