Recent advances in high-throughput quantitative bioanalysis by LC-MS/MS.

  title={Recent advances in high-throughput quantitative bioanalysis by LC-MS/MS.},
  author={Raymond Naxing Xu and Leimin Fan and Matthew J. Rieser and Tawakol A. El-Shourbagy},
  journal={Journal of pharmaceutical and biomedical analysis},
  volume={44 2},

Bioanalytical hydrophilic interaction chromatography: recent challenges, solutions and applications.

Applications of HILIC-MS/MS are summarized, including those for pharmacokinetic studies, metabolic studies, therapeutic drug monitoring and clinical diagnostics, and advancements in the better understanding of retention characteristics of analytes with different mobile- and stationary-phase compositions.

High-performance liquid chromatography–tandem mass spectrometry in the identification and determination of phase I and phase II drug metabolites

Applications of tandem mass spectrometry (MS/MS) techniques coupled with high-performance liquid chromatography (HPLC) in the identification and determination of phase I and phase II drug metabolites

Recent advances in application of hydrophilic interaction chromatography for quantitative bioanalysis.

Hydrophilic interaction chromatography (HILIC) provides a complementary separation mode to RPLC and thus has been gaining increased utilization in LC-MS/MS based quantitative bioanalysis and extended to quantitation of relatively non-polar compounds, peptides and biomarkers.

Quantification of polar drugs in human plasma with liquid chromatography-tandem mass spectrometry.

This review summarizes the recent progress on quantification of polar drugs in plasma with LC-MS/MS andStationary phases of mixed mode and porous graphitic carbon materials are gaining acceptance in bioanalytical applications.

Leveraging Advances in HPLC and Sample Preparation to Maximize DMPK Data Quality

High throughput bioanalytical methods are essential to support the rapid discovery and development of drugs in the pharmaceutical industry and effective sample preparation and appropriate chromatographic separation of endogenous matrix components is required to ensure the quality of the data.

Highly sensitive LC-MS-MS analysis of a pharmaceutical compound in human plasma using monolithic phase-based on-line extraction.

This work has developed a highly sensitive liquid chromatography-tandem mass spectrometry assay for quantitative determination of a pharmaceutical compound in human plasma that exhibited good tolerance to matrix effect and has been applied successfully to a clinical study.

State-of-the-art in fast liquid chromatography-mass spectrometry for bio-analytical applications.

Surface-activated chemical ionization-electrospray ionization mass spectrometry combined with two-dimensional serial chromatography is a powerful tool for drug stability studies.

This work introduces an innovative approach that incorporates two-dimensional liquid chromatography (2D-LC) in conjunction with an MS system consisting of a surface-activated chemical ionization-electrospray ionization chamber (SACI-ESI), an ion trap MS analyzer, and tandem mass spectrometry.



LC-MS Development strategies for quantitative bioanalysis.

Although quantitative bioanalysis using liquid chromatography in conjunction with atmospheric pressure ionization tandem mass spectrometry (LC-MS/MS) has been in use for approximately fifteen years,

Recent advances in use of LC/MS/MS for quantitative high-throughput bioanalytical support of drug discovery.

This review focuses on recent advances in LC/MS/MS based bioanalysis in support of drug discovery and LO, including pooling methods such as cassette dosing along with more highly integrated strategies linking bioanalysis with protocol generation and sample collection.

Column-Switching Procedures for the Fast Analysis of Drugs in Biologic Samples

Different extraction supports allowing the direct injection of biologic fluids were investigated, namely, restricted-access media, large-size particle, and monolithic phases, which were found suitable for the fast analysis of different drugs and their metabolites in biologic matrices at the nanogram per milliliter level.

Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS.

Practical, experimental approaches for studying, identifying, and eliminating the effect of matrix on the results of quantitative analyses by HPLC-MS/MS are described and it is demonstrated that, for the investigational drug under study, the matrix effect was clearly observed when ISP interface was utilized but it was absent when the HN interface was employed.

A Parallel Micro Turbulent Flow Chromatography-Tandem Mass Spectrometry Method for the Analysis of a Pharmaceutical Compound in Plasma

Turbulent flow chromatography coupled to tandem mass spectrometry (TFC-MS-MS) has been widely used within bioanalysis, because of the ability to inject directly neat biological samples with no prior

High-throughput biological sample analysis using on-line turbulent flow extraction combined with monolithic column liquid chromatography/tandem mass spectrometry.

A high-throughput liquid chromatography/tandem mass spectrometry (LC/MS/MS) method, which combines on-line sample extraction through turbulent flow chromatography with a monolithic column separation,

Increasing speed and throughput when using HPLC-MS/MS systems for drug metabolism and pharmacokinetic screening.

Current high throughput strategies and efficient methodologies that are employed for drug metabolism and pharmacokinetic screens for a series of drug discovery compounds, including sample reduction and cassette dosing are summarized.