Recent advances in high-pressure freezing: equipment- and specimen-loading methods.

@article{McDonald2007RecentAI,
  title={Recent advances in high-pressure freezing: equipment- and specimen-loading methods.},
  author={Kent L. McDonald and Mary Morphew and Paul Verkade and Thomas M{\"u}ller-Reichert},
  journal={Methods in molecular biology},
  year={2007},
  volume={369},
  pages={143-73}
}
This chapter is an update of material first published by McDonald in the first volume of this book. Here, we discuss the improvements in the technology and the methodology of high-pressure freezing (HPF) since that article was published. First, we cover the latest innovation in HPF, the Leica EM PACT2. This machine differs significantly from the BAL-TEC HPM 010 high-pressure freezer, which was the main subject of the former chapter. The EM PACT2 is a smaller, portable machine and has an… CONTINUE READING
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