Recent advances in chemical analysis of licorice (Gan-Cao).

  title={Recent advances in chemical analysis of licorice (Gan-Cao).},
  author={Mengzhen Cheng and Jianqing Zhang and Lin Yang and Shijie Shen and Ping Li and Shuai Yao and Hua Qu and Jiayuan Li and Chang-liang Yao and Wen-long Wei and De‐an Guo},
Gan-Cao, or licorice, the dried roots and rhizomes of Glycyrrhiza uralensis, G.glabra, and G.inflata, has received considerable interest due to its extensive application in traditional Chinese medicine (TCM) prescriptions (60% approximately), clinical therapy, and as food additives world-wide. Chemical analysis is an important approach to understand the active pharmaceutical components in licorice and its prescriptions, as well as to develop novel methodologies for their quality assessment and… 
1 Citations
A general procedure for establishing composite quality evaluation indices based on key quality attributes of traditional Chinese medicine.
  • Jia Chen, Xian-Long Cheng, +6 authors Shuang-Cheng Ma
  • Medicine
    Journal of pharmaceutical and biomedical analysis
  • 2021
The results suggested that liquiritin and eight other compounds could be used as quality control indicators of licorice, which provided a foundation to establish the TCM quality composite evaluation index (TCM QCEI).


A new exploration of licorice metabolome.
A chemical characterization of three species of licorice is proposed, through an untargeted metabolomic approach and using high-resolution mass spectrometry, which reveals new possible markers for the analyzed species, and provides a reliable identification of a high number of metabolites, contributing to the characterization of Glycyrrhiza metabolome.
Chemical analysis of the Chinese herbal medicine Gan-Cao (licorice).
This review describes the progress in chemical analysis of licorice and its preparations since 2000, and concludes that the combination of HPLC and LC/MS is currently the most powerful technique for the quality control of Licorice.
Analysis and comparison of Radix Glycyrrhizae (licorice) from Europe and China by capillary-zone electrophoresis (CZE).
G. glabra was distinguished from G. uralensis especially by phenolic compounds GLAB and LQ, and Glycyrrhiza species were also distinguished by applying PCA on the basis of CZE peak area data of GL, GLAB, GA, LQ and LC(A).
Simultaneous Determination of Glycyrrhizin and 15 Flavonoids in Licorice and Blood by High Performance Liquid Chromatography with Ultraviolet Detector
Licorice is the most frequently used herb in traditional Chinese medicine (TCM) with versatile functions. It is also a popular natural dietary supplement. While the dosages is very important for
Metabolites identification of bioactive licorice compounds in rats.
  • Qi Wang, Y. Qian, +7 authors M. Ye
  • Chemistry, Medicine
    Journal of pharmaceutical and biomedical analysis
  • 2015
The metabolites of seven representative bioactive licorice compounds in rats were identified in the plasma, urine and fecal samples, and 22β-acetoxyl glycyrrhizin-6″-methyl ester (1-M2) is a new compound.
Enrichment of total flavones and licochalcone A from licorice residues and its hypoglycemic activity.
The proposed technique is uncomplicated, easily managed, cost-effective, and environmentally friendly and is proper for both large-scale licorice residues application and waste management.
Identification and Chemical Standardization of Licorice Raw Materials and Dietary Supplements Using UHPLC-MS/MS.
An ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) assay using the method of standard addition was developed to quantify 14 licorice components and found that G. glabra, G.uralensis, and G.inflata in a variety of forms including root powders and extracts as well as complex dietary supplements could be differentiated and chemically standardized without concerns due to matrix effects.
Liquorice, a unique "guide drug" of traditional Chinese medicine: a review of its role in drug interactions.
The overview and comparison of traditional uses of liquorice with recent pharmacological studies and randomized controlled trials provide new insights into this ancient drug for future investigations and clinical use, especially in drug combination.
Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS.
A rapid and sensitive method based on ultra high-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight mass spectrometry (UHPLC-DAD-qTOF-MS) was established to characterize the chemical constituents and rats metabolites of GQW.
Quantification of four active ingredients and fingerprint analysis of Licorice (Glycyrrhiza uralensis Fisch.) after spaceflight by HPLC–DAD
The report provided the scientific data for spaceflight breeding of a medicinal plant and indicated that extraterrestrial orbit is a possible way in accelerating the rate of breeding and select eximious germplasm resources.