RecJ exonuclease: substrates, products and interaction with SSB

  title={RecJ exonuclease: substrates, products and interaction with SSB},
  author={Eugene S. Han and Deani L. Cooper and Nicole S. Persky and Vincent A. Sutera and Richard D. Whitaker and Melissa L. Montello and Susan T Lovett},
  journal={Nucleic Acids Research},
  pages={1084 - 1091}
The RecJ exonuclease from Escherichia coli degrades single-stranded DNA (ssDNA) in the 5'-3' direction and participates in homologous recombination and mismatch repair. The experiments described here address RecJ's substrate requirements and reaction products. RecJ complexes on a variety of 5' single-strand tailed substrates were analyzed by electrophoretic mobility shift in the absence of Mg2+ ion required for substrate degradation. RecJ required single-stranded tails of 7 nt or greater for… CONTINUE READING


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