RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue

@inproceedings{Manosas2013RecGAU,
  title={RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue},
  author={Maria Manosas and Senthil K. Perumal and Piero R. Bianco and Felix Ritort and Stephen J Benkovic and Vincent Croquette},
  booktitle={Nature communications},
  year={2013}
}
Helicases that both unwind and rewind DNA have central roles in DNA repair and genetic recombination. In contrast to unwinding, DNA rewinding by helicases has proved difficult to characterize biochemically because of its thermodynamically downhill nature. Here we use single-molecule assays to mechanically destabilize a DNA molecule and follow, in real time, unwinding and rewinding by two DNA repair helicases, bacteriophage T4 UvsW and Escherichia coli RecG. We find that both enzymes are robust… CONTINUE READING
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