Reassuringly low carriage of enteropathogens among healthy Swedish children in day care centres.

Abstract

OBJECTIVES Infectious gastroenteritis is one of the most common diseases among children and has a considerable impact on health and socio-economy. Day care centres are high-risk environments for infections. The aim of this study was to investigate if asymptomatic preschool children constitute a reservoir for potential enteropathogens. STUDY DESIGN In total, 438 individual diapers were collected from day care centres in Uppsala, Sweden, during spring and autumn, and molecular techniques were used to estimate the prevalence of asymptomatic carriage of multiple enteropathogens. METHODS Faecal samples were analysed with multiplex polymerase chain reaction (PCR) (xTAG® Gastrointestinal Pathogen Panel; Luminex Corporation, Toronto, Canada) targeting 21 different pathogens. Samples with a median fluorescence intensity above threshold were re-analysed with a second PCR assay. RESULTS Sixteen of the 438 samples were positive for enteropathogens, 1.6% for enteric adenovirus, 0.7% for Campylobacter spp., and 0.7% for norovirus. CONCLUSIONS Preschool children in Uppsala constitute a limited reservoir for potential enteropathogens.

DOI: 10.1016/j.puhe.2016.05.011

Cite this paper

@article{Kaarme2016ReassuringlyLC, title={Reassuringly low carriage of enteropathogens among healthy Swedish children in day care centres.}, author={Johan Kaarme and Rachel A. Hickman and Tryggve Nev{\'e}us and Jonas Blomberg and Christina {\"{O}hrmalm}, journal={Public health}, year={2016}, volume={140}, pages={221-227} }