Reassessing the role of the honeybee (Apis mellifera) Dufour's gland in egg marking

  title={Reassessing the role of the honeybee (Apis mellifera) Dufour's gland in egg marking},
  author={Stephen J Martin and Graeme R. Jones and Nicolas Ch{\^a}line and Helen Middleton and Francis L. W. Ratnieks},
Dufour's gland secretion may allow worker honeybees to discriminate between queen-laid and worker-laid eggs. To investigate this, we combined the chemical analysis of individually treated eggs with an egg removal bioassay. We partitioned queen Dufour's gland into hydrocarbon and ester fractions. The bioassay showed that worker-laid eggs treated with either whole gland extract, ester fraction or synthetic gland esters were removed more slowly than untreated worker-laid eggs. However, the effect… CONTINUE READING
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