Realtime PCR Is More Sensitive than Multiplex PCR for Diagnosis and Serotyping in Children with Culture Negative Pneumococcal Invasive Disease

@inproceedings{Azzari2010RealtimePI,
  title={Realtime PCR Is More Sensitive than Multiplex PCR for Diagnosis and Serotyping in Children with Culture Negative Pneumococcal Invasive Disease},
  author={Chiara Azzari and Maria Moriondo and Giuseppe Indolfi and Martina Cortimiglia and Clementina Canessa and Laura Becciolini and Francesca Lippi and Maurizio de Martino and Massimo Resti},
  booktitle={PloS one},
  year={2010}
}
BACKGROUND Pneumococcal serotyping is usually performed by Quellung reaction, considered the gold standard test. However the method cannot be used on culture-negative samples. Molecular methods can be a useful alternative. The aim of the study was to evaluate the use of Multiplex-sequential-PCR (MS-PCR) or Realtime-PCR on blood samples for diagnosis and serotyping of invasive pneumococcal disease (IPD) in a pediatric clinical setting. METHODOLOGY/PRINCIPAL FINDINGS Sensitivity and specificity… CONTINUE READING

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Community‐acquired bacteremic pneumococcal pneumonia in children: diagnosis and serotyping by real‐time polymerase chain reaction using blood samples.

  • Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 2010
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Surveillance for invasive Streptococcus pneumoniae disease among hospitalized children in Bangladesh: antimicrobial susceptibility and serotype distribution.

  • Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 2009
VIEW 1 EXCERPT