Real-time single-cell response to stiffness.

  title={Real-time single-cell response to stiffness.},
  author={D{\'e}mosth{\`e}ne Mitrossilis and Jonathan Fouchard and David P. Pereira and François Postic and Alain Richert and Michel Saint-Jean and Atef Asnacios},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={107 38},
Living cells adapt to the stiffness of their environment. However, cell response to stiffness is mainly thought to be initiated by the deformation of adhesion complexes under applied force. In order to determine whether cell response was triggered by stiffness or force, we have developed a unique method allowing us to tune, in real time, the effective stiffness experienced by a single living cell in a uniaxial traction geometry. In these conditions, the rate of traction force buildup dF/dt was… CONTINUE READING