Real-time single cell analysis of Smac/DIABLO release during apoptosis

@article{Rehm2003RealtimeSC,
  title={Real-time single cell analysis of Smac/DIABLO release during apoptosis},
  author={Markus Rehm and Heiko D{\"u}ssmann and Jochen H. M. Prehn},
  journal={The Journal of Cell Biology},
  year={2003},
  volume={162},
  pages={1031 - 1043}
}
We examined the temporal and causal relationship between Smac/DIABLO release, cytochrome c (cyt-c) release, and caspase activation at the single cell level during apoptosis. Cells treated with the broad-spectrum caspase inhibitor z-VAD-fmk, caspase-3 (Casp-3)-deficient MCF-7 cells, as well as Bax-deficient DU-145 cells released Smac/DIABLO and cyt-c in response to proapoptotic agents. Real-time confocal imaging of MCF-7 cells stably expressing Smac/DIABLO-yellow fluorescent protein (YFP… CONTINUE READING

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