Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis.

@article{Bustin2005RealtimeRT,
  title={Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis.},
  author={Stephen A. Bustin and Reinhold D. Mueller},
  journal={Clinical science},
  year={2005},
  volume={109 4},
  pages={
          365-79
        }
}
qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for the detection of viral load and therapy monitoring, and the development of SARS (severe acute… 
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