Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome.

Abstract

Here we designed and tested two highly specific quantitative TaqMan(®)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).

DOI: 10.1016/j.mcp.2015.09.001

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@article{Douglas2015RealtimeRT, title={Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome.}, author={Christina Elizabeth Douglas and David A. Kulesh and James G. Jaissle and Timothy D Minogue}, journal={Molecular and cellular probes}, year={2015}, volume={29 6}, pages={511-513} }