Real-time reaction monitoring by probe electrospray ionization mass spectrometry.

@article{Yu2010RealtimeRM,
  title={Real-time reaction monitoring by probe electrospray ionization mass spectrometry.},
  author={Zhan Yu and Lee Chuin Chen and Rosa Erra-Balsells and Hiroshi Nonami and Kenzo Hiraoka},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2010},
  volume={24 11},
  pages={1507-13}
}
Probe electrospray ionization (PESI) is a modified version of the electrospray ionization (ESI), where the capillary for sampling and spraying is replaced by a solid needle. High tolerance to salts and direct ambient sampling are major advantages of PESI compared with conventional ESI. In this study, PESI-MS was used to monitor some biological and chemical reactions in real-time, such as acid-induced protein denaturation, hydrogen/deuterium exchange (HDX) of peptides, and Schiff base formation… CONTINUE READING