Real-time quantitative polymerase chain reaction (QPCR) to identify Myxobolus cerebralis in rainbow trout Oncorhynchus mykiss.

Abstract

This study describes the development of a TaqMan real-time quantitative polymerase chain reaction (QPCR) technique using the heat-shock protein 70 (Hsp 70) and 18S ribosomal DNA (18S rDNA) sequences to identify Myxobolus cerebralis and attempt to quantify infection severity within rainbow trout fry Oncorhynchus mykiss. Rainbow trout for this study were… (More)

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Cite this paper

@article{Cavender2004RealtimeQP, title={Real-time quantitative polymerase chain reaction (QPCR) to identify Myxobolus cerebralis in rainbow trout Oncorhynchus mykiss.}, author={Wade P Cavender and John Stephen Wood and Madison S. Powell and Kenneth E Overturf and Kenneth D. Cain}, journal={Diseases of aquatic organisms}, year={2004}, volume={60 3}, pages={205-13} }