Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.

@article{Zucol2006RealtimeQB,
  title={Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.},
  author={Franziska Zucol and Roland A. Ammann and Christoph Berger and Christoph Aebi and Martin Altwegg and Felix K. Niggli and David Nadal},
  journal={Journal of clinical microbiology},
  year={2006},
  volume={44 8},
  pages={2750-9}
}
Here we determined the analytical sensitivities of broad-range real-time PCR-based assays employing one of three different genomic DNA extraction protocols in combination with one of three different primer pairs targeting the 16S rRNA gene to detect a panel of 22 bacterial species. DNA extraction protocol III, using lysozyme, lysostaphin, and proteinase K… CONTINUE READING