Real-time quantitative RT-PCR assay of GADD45 gene expression changes as a biomarker for radiation biodosimetry.

@article{Grace2002RealtimeQR,
  title={Real-time quantitative RT-PCR assay of GADD45 gene expression changes as a biomarker for radiation biodosimetry.},
  author={Marcy B Grace and Christopher B McLeland and William F. Blakely},
  journal={International journal of radiation biology},
  year={2002},
  volume={78 11},
  pages={1011-21}
}
PURPOSE To assess the efficacy of fluorescent-based quantitative reverse transcription-polymerase chain reaction (QRT-PCR) technology to measure gene expression changes (GEC) for rapid, point-of-care radiation dose assessment. MATERIALS AND METHODS A real-time QRT-PCR assay based on 5'-fluorogenic nuclease TaqMan(TM) methodology was developed, which employs both relative and absolute quantification of a candidate mRNA biomarker. Growth arrest and DNA damage gene 45 (GADD45), a cell-cycle… CONTINUE READING

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