Real time quantitative PCR.

  title={Real time quantitative PCR.},
  author={Christopher Heid and Janie Stevens and Kenneth J Livak and P. Mickey Williams},
  journal={Genome research},
  volume={6 10},
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a… CONTINUE READING
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