Real-time quantitative PCR of microdissected paraffin-embedded breast carcinoma: an alternative method for HER-2/neu analysis.

@article{Gjerdrum2004RealtimeQP,
  title={Real-time quantitative PCR of microdissected paraffin-embedded breast carcinoma: an alternative method for HER-2/neu analysis.},
  author={Lise Mette Rahbek Gjerdrum and Boe Sandahl Sorensen and Eigil Kjeldsen and Flemming Brandt Sorensen and Ebba Nexo and Stephen Jacques Hamilton-Dutoit},
  journal={The Journal of molecular diagnostics : JMD},
  year={2004},
  volume={6 1},
  pages={42-51}
}
We studied the feasibility of using real-time quantitative PCR to determine HER-2 DNA amplification and mRNA expression in microdissected formalin-fixed, paraffin-embedded breast tumors and compared this with standard immunohistochemistry (IHC) and fluorescent in situ hybridization (FISH) methods. Study cases (27 carcinomas and 3 ductal breast carcinoma in situ (DCIS) cases) showed varying Her-2 expression as determined by IHC (HercepTest). In carcinomas, there was a good correlation between… CONTINUE READING

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