Real-time quantitative PCR for detection of Helicobacter pylori.

  title={Real-time quantitative PCR for detection of Helicobacter pylori.},
  author={Qiang He and Jian Ping Wang and Michael S. Osato and Lawrence B. Lachman},
  journal={Journal of clinical microbiology},
  volume={40 10},
Helicobacter pylori is one of the most common chronic infections in humans, in whom it is a key etiological factor in peptic ulcer disease, gastric mucosa-associated lymphoid tissue lymphoma, and gastric adenocarcinoma. Humans are the bacterium's only host. Here we report the development of a real-time quantitative (Q) PCR-based assay to measure ureC gene copy number to detect H. pylori, based on the fact that there is only one copy of the ureC gene per bacterium. Upon optimization of… CONTINUE READING


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